New Publication Presents Bluues2, the Enhanced Successor to Bluues 

5 October 2026
31 October 2026
1–2 minutes

BioComputing UP is pleased to share the publication of the article “Bluues2: An Enhanced Web Server for Fast and Accurate Electrostatic Analysis of Proteins and Protein Complexes” in Protein Science, as part of the special issue “Tools for Protein Science.”
Read the article: https://onlinelibrary.wiley.com/doi/10.1002/pro.70815

The paper presents Bluues2, an enhanced web server for the analysis of protein electrostatic properties. The platform introduces improved accuracy and new functionalities for studying protein–protein and protein–ligand complexes, including the calculation of binding free energies and electrostatic complementarity. Additional features include an integrated 3D structure viewer and a redesigned user interface, providing researchers with a more accessible and efficient environment for electrostatic analyses.

The Bluues2 server is accessible at https://bluues.biocomputingup.it/.

The redesigned Bluues2 interface, providing access to electrostatic analyses and visualization of protein structures and molecular complexes. 

Bluues2 can be used to explore electrostatic properties and interaction features of molecular complexes through both visual and numerical analyses. The figure below illustrates representative results obtained with the server for the p27Kip1–cyclin A–Cdk2 complex.

Representative Bluues2 results for the p27Kip1–cyclin A–Cdk2 complex (PDB ID: 1JSU), including the 3D structure, numerical analysis results, and partner-facing interface surfaces generated through complex-mode analysis.

The work was carried out by Damiano Piovesan, Nima Rasekh, Miguel Angel Soler, Walter Rocchia, Gennaro Esposito, Federico Fogolari, and Silvio C. E. Tosatto.

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